More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Rad50 Rabbit pAb

Rad50 Rabbit pAb

A0182
ABclonal
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetRAD50
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    ABclonal
  • Product Name
    Rad50 Rabbit pAb
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB,IHC,IF/ICC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10111
  • Target name
    RAD50
  • Target description
    RAD50 double strand break repair protein
  • Target synonyms
    NBSLD, RAD502, hRad50, DNA repair protein RAD50, RAD50 homolog, double strand break repair protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92878
  • Protein Name
    DNA repair protein RAD50
  • Reactivity
    Human, Mouse
  • Reactivity Supplier Note
    Human,Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Assay Genie
Rad50 AntibodyCAB0182
ReactivityHuman
TargetRAD50
  • SizePrice
Western Blot analysis of COLO205 cells using Rad50 Polyclonal Antibody
Product group Antibodies
Cusabio
RAD50 AntibodyCSB-PA003915
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetRAD50
  • SizePrice
Figure 1. Western blot analysis of RAD50 using anti-RAD50 antibody (A00347-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: human Caco-2 whole cell lysates, Lane 7: human Daudi whole cell lysates, Lane 8: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAD50 antigen affinity purified polyclonal antibody (Catalog # A00347-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAD50 at approximately 154 kDa. The expected band size for RAD50 is at 154 kDa.
Product group Antibodies
Boster Bio
Anti-RAD50 Antibody Picoband(r)A00347-2-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetRAD50
  • SizePrice
Product group Antibodies
RayBiotech
Anti-RAD50 Antibody144-03078
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman, Mouse, Rat
TargetRAD50
  • SizePrice
Anti-RAD50 Antibody
Product group Antibodies
Antibodies.com
Anti-RAD50 AntibodyA97304
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
  • SizePrice
Product group Antibodies
MedChem Express
Rad50 Antibody (YA1681, Rabbit)HY-P81936
TargetRAD50
  • SizePrice
Immunohistochemical staining of human skin shows strong nuclear positivity in squamous epithelial cells.
Product group Antibodies
Atlas Antibodies
Anti-RAD50 AntibodyHPA052291
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetRAD50
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
RAD50 AntibodyLS-C402529
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetRAD50
  • SizePrice
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [N1N2], N-term (GTX119731) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
GeneTex
Rad50 antibody [N1N2], N-termGTX119731
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetRAD50
  • SizePrice
Product group Antibodies
Bioss
RAD50 Recombinant Antibody, AbBy Fluor-488 ConjugatedBSM-61559R-BF488
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetRAD50
  • SizePrice