
ICC/IF analysis of U2OS 40-2-6 cells treated with or without 2 mM hydroxyurea (HU) for 24 hrs using GTX00884 Rad52 antibody. The cells were pre-extracted with 0.1% Triton X-100 in PBS, fixed with 4% PFA in PBS. Triton X-100 treatment extracts chromatin-free Rad52 from nuclei. Scale bars : 20 μm Dilution : 1:50
Rad52 antibody
GTX00884
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityHamster, Human, Mouse, Rat
TargetRAD52
Overview
- SupplierGeneTex
- Product NameRad52 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:3000. ICC/IF: 1:1000. IP: 1:50-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID5893
- Target nameRAD52
- Target descriptionRAD52 DNA repair protein
- Target synonymsDNA repair protein RAD52 homolog, RAD52 homolog, DNA repair protein, recombination protein RAD52, rhabdomyosarcoma antigen MU-RMS-40.23
- HostRabbit
- IsotypeIgG
- Protein IDP43351
- Protein NameDNA repair protein RAD52 homolog
- Scientific DescriptionThe protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. A pseudogene of this gene is present on chromosome 2. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jul 2014]
- ReactivityHamster, Human, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161










![Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Rad52 antibody [5H9] (GTX70301) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX70301/GTX70301_44923_20230113_WB_23011618_845.webp)
