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Radixin antibody

GTX105408
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
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Product group Antibodies
TargetRDX
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Overview

  • Supplier
    GeneTex
  • Product Name
    Radixin antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.9 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5962
  • Target name
    RDX
  • Target description
    radixin
  • Target synonyms
    DFNB24; radixin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35241
  • Protein Name
    Radixin
  • Scientific Description
    Radixin is a cytoskeletal protein that may be important in linking actin to the plasma membrane. It is highly similar in sequence to both ezrin and moesin. The radixin gene has been localized by fluorescence in situ hybridization to 11q23. A truncated version representing a pseudogene (RDXP2) was assigned to Xp21.3. Another pseudogene that seemed to lack introns (RDXP1) was mapped to 11p by Southern and PCR analyses. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Moesin Serves as Scaffold Protein for PD-L1 in Human Uterine Cervical Squamous Carcinoma Cells. Doukuni R et al., 2022 Jul 1, J Clin Med
    Read more
  • Ezrin Contributes to the Plasma Membrane Expression of PD-L1 in A2780 Cells. Tameishi M et al., 2022 Apr 27, J Clin Med
    Read more
  • Ezrin Regulates the Cell Surface Localization of PD-L1 in HEC-151 Cells. Tanaka C et al., 2022 Apr 15, J Clin Med
    Read more
  • Contribution of Ezrin on the Cell Surface Plasma Membrane Localization of Programmed Cell Death Ligand-1 in Human Choriocarcinoma JEG-3 Cells. Tameishi M et al., 2021 Sep 24, Pharmaceuticals (Basel)
    Read more
  • Role of Ezrin/Radixin/Moesin in the Surface Localization of Programmed Cell Death Ligand-1 in Human Colon Adenocarcinoma LS180 Cells. Kobori T et al., 2021 Aug 28, Pharmaceuticals (Basel)
    Read more
  • Ezrin Modulates the Cell Surface Expression of Programmed Cell Death Ligand-1 in Human Cervical Adenocarcinoma Cells. Tanaka C et al., 2021 Sep 17, Molecules
    Read more
  • Subcellular distribution of ezrin/radixin/moesin and their roles in the cell surface localization and transport function of P-glycoprotein in human colon adenocarcinoma LS180 cells. Kobori T et al., 2021, PLoS One
    Read more
  • Resources for the Comprehensive Discovery of Functional RNA Elements. Sundararaman B et al., 2016 Mar 17, Mol Cell
    Read more
  • A Dual Role for Melatonin in Medaka Ovulation: Ensuring Prostaglandin Synthesis and Actin Cytoskeleton Rearrangement in Follicular Cells. Ogiwara K et al., 2016 Mar, Biol Reprod
    Read more
  • Talin regulates moesin-NHE-1 recruitment to invadopodia and promotes mammary tumor metastasis. Beaty BT et al., 2014 Jun 9, J Cell Biol
    Read more

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Figure 1. Western blot analysis of RDX using anti-RDX antibody (A01926-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human U2OS whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human A431 whole cell lysates, Lane 6: human PC-3 whole cell lysates, Lane 7: human HepG2 whole cell lysates, Lane 8: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RDX antigen affinity purified polyclonal antibody (Catalog # A01926-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RDX at approximately 88KD. The expected band size for RDX is at 69KD.
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