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Western Blot analysis of HuvEc cells using Radixin Polyclonal Antibody
Western Blot analysis of HuvEc cells using Radixin Polyclonal Antibody
Western Blot analysis of HuvEc cells using Radixin Polyclonal Antibody

RDX Antibody

CSB-PA003919
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetRDX
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Overview

  • Supplier
    Cusabio
  • Product Name
    RDX Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5962
  • Target name
    RDX
  • Target description
    radixin
  • Target synonyms
    DFNB24; radixin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35241
  • Protein Name
    Radixin
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RDX using anti-RDX antibody (A01926-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human U2OS whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human A431 whole cell lysates, Lane 6: human PC-3 whole cell lysates, Lane 7: human HepG2 whole cell lysates, Lane 8: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RDX antigen affinity purified polyclonal antibody (Catalog # A01926-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RDX at approximately 88KD. The expected band size for RDX is at 69KD.
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