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Western blot All lanes: RAE1 antibody at 4.39ug/ml Lane 1: Mouse brain tissue Lane 2: HepG2 whole cell lysate Lane 3: 293T whole cell lysate Lane 4: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 41 kDa Observed band size: 41, 80 kDa
Western blot All lanes: RAE1 antibody at 4.39ug/ml Lane 1: Mouse brain tissue Lane 2: HepG2 whole cell lysate Lane 3: 293T whole cell lysate Lane 4: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 41 kDa Observed band size: 41, 80 kDa
Western blot All lanes: RAE1 antibody at 4.39ug/ml Lane 1: Mouse brain tissue Lane 2: HepG2 whole cell lysate Lane 3: 293T whole cell lysate Lane 4: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 41 kDa Observed band size: 41, 80 kDa

RAE1 Antibody

CSB-PA019276ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetRAE1
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Overview

  • Supplier
    Cusabio
  • Product Name
    RAE1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8480
  • Target name
    RAE1
  • Target description
    ribonucleic acid export 1
  • Target synonyms
    dJ481F12.3; dJ800J21.1; Gle2; homolog of yeast Rae1 (Bharathi) mRNA-associated protein of 41 kDa (Kraemer); MIG14; migration-inducing gene 14; Mnrp41; mRNA export factor; mRNA export protein; mRNA-associated protein MRNP 41; mRNA-binding protein, 41-kD; MRNP41; rae1 protein homolog; RAE1 RNA export 1 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP78406
  • Protein Name
    mRNA export factor
  • Scientific Description
    Binds mRNA. May function in nucleocytoplasmic transport and in directly or indirectly attaching cytoplasmic mRNPs to the cytoskeleton.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RAE1 using anti-RAE1 antibody (A04228-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: rat thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAE1 antigen affinity purified polyclonal antibody (Catalog # A04228-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAE1 at approximately 41 kDa. The expected band size for RAE1 is at 41 kDa.
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