Untreated (-) and treated (+) 293T whole cell extracts (30 microg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Raf1 (phospho Ser259) antibody [GT1309] (GTX03221) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
![WB analysis of 293T cells were treated by Forskolin (10 uM) at 37oC for 1 hour after serum-starvation overnight using GTX03221 Raf1 (phospho Ser259) antibody [GT1309]. Dilution : 1:1000 Loading : 25microg per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX03221/GTX03221_80_WB_w_23053123_762.webp "WB analysis of 293T cells were treated by Forskolin (10 uM) at 37oC for 1 hour after serum-starvation overnight using GTX03221 Raf1 (phospho Ser259) antibody [GT1309]. Dilution : 1:1000 Loading : 25microg per lane")
![WB analysis of C6 cells were treated by Forskolin (10 uM) at 37oC for 1 hour after serum-starvation overnight using GTX03221 Raf1 (phospho Ser259) antibody [GT1309]. Dilution : 1:1000 Loading : 25microg per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX03221/GTX03221_81_WB_w_23053123_624.webp "WB analysis of C6 cells were treated by Forskolin (10 uM) at 37oC for 1 hour after serum-starvation overnight using GTX03221 Raf1 (phospho Ser259) antibody [GT1309]. Dilution : 1:1000 Loading : 25microg per lane")
Untreated (-) and treated (+) 293T whole cell extracts (30 microg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Raf1 (phospho Ser259) antibody [GT1309] (GTX03221) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Raf1 (phospho Ser259) antibody [GT1309] - Orthogonal Validated
GTX03221
Overview
- SupplierGeneTex
- Product NameRaf1 (phospho Ser259) antibody [GT1309] - Orthogonal Validated
- Delivery Days Customer9
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT1309
- Concentration1.25 mg/ml
- ConjugateUnconjugated
- Gene ID5894
- Target nameRAF1
- Target descriptionRaf-1 proto-oncogene, serine/threonine kinase
- Target synonymsCMD1NN; CRAF; c-Raf; C-Raf proto-oncogene, serine/threonine kinase; NS5; Oncogene RAF1; proto-oncogene c-RAF; raf proto-oncogene serine/threonine protein kinase; RAF proto-oncogene serine/threonine-protein kinase; Raf-1; v-raf-1 murine leukemia viral oncogene homolog 1; v-raf-1 murine leukemia viral oncogene-like protein 1
- HostRabbit
- IsotypeIgG
- Modification TypePhosphorylated
- Scientific DescriptionThis gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq, Jul 2008]
- ReactivityHuman, Rat
- Storage Instruction2°C to 8°C,-20°C or -80°C
- UNSPSC12352203