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Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA019296LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA019296LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA019296LA01HU at dilution of 1:100

RALA Antibody

CSB-PA019296LA01HU
Cusabio
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetRALA
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Overview

  • Supplier
    Cusabio
  • Product Name
    RALA Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5898
  • Target name
    RALA
  • Target description
    RAS like proto-oncogene A
  • Target synonyms
    HINCONS; RAL; RALA Ras like proto-oncogene A; Ras family small GTP binding protein RALA; ras related GTP binding protein A; RAS-like protein A; ras-related protein Ral-A; v-ral simian leukemia viral oncogene homolog A (ras related)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP11233
  • Protein Name
    Ras-related protein Ral-A
  • Scientific Description
    Multifunctional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. Plays a role in the early stages of cytokinesis and is required to tether the exocyst to the cytokinetic furrow. The RALA-exocyst complex regulates integrin-dependent membrane raft exocytosis and growth signaling. Key regulator of LPAR1 signaling and competes with GRK2 for binding to LPAR1 thus affecting the signaling properties of the receptor. Required for anchorage-independent proliferation of transformed cells.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RALA using anti-RALA antibody (PB9794). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human SW620 whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RALA antigen affinity purified polyclonal antibody (Catalog # PB9794) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RALA at approximately 24 kDa. The expected band size for RALA is at 24 kDa.
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