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Anti-RALA Antibody

CAB0541
Assay Genie
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityMouse
TargetRALA
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Overview

  • Supplier
    Assay Genie
  • Product Name
    Anti-RALA Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB,IF
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    Not applicable
  • Conjugate
    Unconjugated
  • Gene ID5898
  • Target name
    RALA
  • Target description
    RAS like proto-oncogene A
  • Target synonyms
    HINCONS, RAL, ras-related protein Ral-A, RALA Ras like proto-oncogene A, RAS-like protein A, Ras family small GTP binding protein RALA, ras related GTP binding protein A, v-ral simian leukemia viral oncogene homolog A (ras related)
  • Host
    Rabbit
  • Protein IDP11233
  • Protein Name
    Ras-related protein Ral-A
  • Scientific Description
    RALA Antibody is a premium polyclonal antibody that offers outstanding performance and reliability for demanding research applications. Rigorously validated for WB, ELISA, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Mouse,Rat samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 100microL format to meet your experimental needs. For optimal performance, store at -20°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Mouse
  • Reactivity Supplier
    Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RALA using anti-RALA antibody (PB9794). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human SW620 whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RALA antigen affinity purified polyclonal antibody (Catalog # PB9794) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RALA at approximately 24 kDa. The expected band size for RALA is at 24 kDa.
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