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Western Blot Positive WB detected in: K562 whole cell lysate, HEK293 whole cell lysate, MCF-7 whole cell lysate All lanes: RALBP1 antibody at 4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 77 kDa Observed band size: 77 kDa
Western Blot Positive WB detected in: K562 whole cell lysate, HEK293 whole cell lysate, MCF-7 whole cell lysate All lanes: RALBP1 antibody at 4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 77 kDa Observed band size: 77 kDa
Western Blot Positive WB detected in: K562 whole cell lysate, HEK293 whole cell lysate, MCF-7 whole cell lysate All lanes: RALBP1 antibody at 4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 77 kDa Observed band size: 77 kDa

RALBP1 Antibody

CSB-PA618981LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRALBP1
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Overview

  • Supplier
    Cusabio
  • Product Name
    RALBP1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10928
  • Target name
    RALBP1
  • Target description
    ralA binding protein 1
  • Target synonyms
    76 kDa Ral-interacting protein; dinitrophenyl S-glutathione ATPase; DNP-SG ATPase; ralA-binding protein 1; ral-interacting protein 1; RIP1; RLIP1; RLIP76
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15311
  • Protein Name
    RalA-binding protein 1
  • Scientific Description
    Can activate specifically hydrolysis of GTP bound to RAC1 and CDC42, but not RALA. Mediates ATP-dependent transport of S-(2,4-dinitrophenyl)-glutathione (DNP-SG) and doxorubicin (DOX) and is the major ATP-dependent transporter of glutathione conjugates of electrophiles (GS-E) and DOX in erythrocytes. Can catalyze transport of glutathione conjugates and xenobiotics, and may contribute to the multidrug resistance phenomenon. Serves as a scaffold protein that brings together proteins forming an endocytotic complex during interphase and also with CDK1 to switch off endocytosis, One of its substrates would be EPN1/Epsin.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of RALBP1 using anti-RALBP1 antibody (A01403-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human SGC-7901 whole cell lysates, Lane 5: human 22RV1 whole cell lysates, Lane 6: rat lung tissue lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse testis tissue lysates, Lane 9: mouse ovary tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RALBP1 antigen affinity purified polyclonal antibody (Catalog # A01403-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RALBP1 at approximately 95KD. The expected band size for RALBP1 is at 76KD.
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