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RALBP1 Antibody (YA3053, Rabbit)

HY-P83308
MedChem Express
TargetRALBP1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    RALBP1 Antibody (YA3053, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID10928
  • Target name
    RALBP1
  • Target description
    ralA binding protein 1
  • Target synonyms
    RIP1, RLIP1, RLIP76, ralA-binding protein 1, 76 kDa Ral-interacting protein, DNP-SG ATPase, dinitrophenyl S-glutathione ATPase, ral-interacting protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    RALBP1 Antibody (YA3053) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA3053), targeting RALBP1, with a predicted molecular weight of 76 kDa (observed band size: 95 kDa). RALBP1 Antibody (YA3053) can be used for WB, IHC-P, FC experiment in human, mouse, rat background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RALBP1 using anti-RALBP1 antibody (A01403-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human SGC-7901 whole cell lysates, Lane 5: human 22RV1 whole cell lysates, Lane 6: rat lung tissue lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse testis tissue lysates, Lane 9: mouse ovary tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RALBP1 antigen affinity purified polyclonal antibody (Catalog # A01403-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RALBP1 at approximately 95KD. The expected band size for RALBP1 is at 76KD.
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