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ProductsBack/RanBP2 Polyclonal Antibody
Antigen: bs-9323P, 2ug/100ul \nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \nSecondary: HRP conjugated Rabbit Anti-Goat IgG(bs-0294R-HRP) at 1: 5000; \nTMB staining\nRead the data in Microplate Reader by 450nm. \n
Antigen: bs-9323P, 2ug/100ul \nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \nSecondary: HRP conjugated Rabbit Anti-Goat IgG(bs-0294R-HRP) at 1: 5000; \nTMB staining\nRead the data in Microplate Reader by 450nm. \n
Antigen: bs-9323P, 2ug/100ul \nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \nSecondary: HRP conjugated Rabbit Anti-Goat IgG(bs-0294R-HRP) at 1: 5000; \nTMB staining\nRead the data in Microplate Reader by 450nm. \n

RanBP2 Polyclonal Antibody

BS-9323R
Bioss
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat, Other Species
TargetRANBP2
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Overview

  • Supplier
    Bioss
  • Product Name
    RanBP2 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA(1:500-1000), IHC-P(1:200-400), IF(IHC-P)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID5903
  • Target name
    RANBP2
  • Target description
    RAN binding protein 2
  • Target synonyms
    ADANE, ANE1, IIAE3, NUP358, TRP1, TRP2, E3 SUMO-protein ligase RanBP2, 358 kDa nucleoporin, E3 SUMO-protein transferase RanBP2, P270, acute necrotizing encephalopathy 1 (autosomal dominant), nuclear pore complex protein Nup358, nucleoporin 358, nucleoporin Nup358, ran-binding protein 2, transformation-related protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat, Other Species
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RANBP2 using anti-RANBP2 antibody (A00981-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RANBP2 antigen affinity purified polyclonal antibody (Catalog # A00981-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RANBP2 at approximately 358 kDa. The expected band size for RANBP2 is at 358 kDa.
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