![Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAS (G12D Mutant) antibody [HL2640] (GTX639096) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAS (G12D Mutant) antibody [HL2640] (GTX639096) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX639096/GTX639096_45257_20231215_WB_23121922_250.webp)
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAS (G12D Mutant) antibody [HL2640] (GTX639096) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
RAS (G12D Mutant) antibody [HL2640]
GTX639096
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetKRAS
Overview
- SupplierGeneTex
- Product NameRAS (G12D Mutant) antibody [HL2640]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2640
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID3845
- Target nameKRAS
- Target descriptionKRAS proto-oncogene, GTPase
- Target synonyms'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, K-Ras 2, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RASK2, c-Ki-ras, c-Ki-ras2, GTPase KRas, K-ras p21 protein, KRAS-ENOPH fusion, Kirsten rat sarcoma viral oncogene homolog, Kirsten rat sarcoma viral proto-oncogene, Kras-Enoph1 fusion protein, PR310 c-K-ras oncogene, c-Kirsten-ras protein, cellular c-Ki-ras2 proto-oncogene, cellular transforming proto-oncogene, oncogene KRAS2, proto-oncogene GTPase, transforming protein p21, v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog
- HostRabbit
- IsotypeIgG
- Protein IDP01116
- Protein NameGTPase KRas
- Scientific DescriptionThis gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![RAS (G12D Mutant) antibody [HL2640] detects RAS (G12D Mutant) protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min. Green: RAS (G12D Mutant) stained by RAS (G12D Mutant) antibody [HL2640] (GTX639096) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). RAS (G12D Mutant) antibody [HL2640] detects RAS (G12D Mutant) protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min. Green: RAS (G12D Mutant) stained by RAS (G12D Mutant) antibody [HL2640] (GTX639096) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX639096/GTX639096_T-45187_20231124_ICC_IF_B_23121922_372.webp)
![RAS (G12D Mutant) antibody [HL2640] detects RAS (G12D Mutant) protein by immunofluorescent analysis. Sample: BxPC3 (RAS WT) and AsPC-1 (KRAS G12D) cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RAS (G12D Mutant) stained by RAS (G12D Mutant) antibody [HL2640] (GTX639096) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). RAS (G12D Mutant) antibody [HL2640] detects RAS (G12D Mutant) protein by immunofluorescent analysis. Sample: BxPC3 (RAS WT) and AsPC-1 (KRAS G12D) cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RAS (G12D Mutant) stained by RAS (G12D Mutant) antibody [HL2640] (GTX639096) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX639096/GTX639096_45257_20250523_ICC_IF_25061300_832.webp)
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAS (G12D Mutant) antibody [HL2640] (GTX639096) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAS (G12D Mutant) antibody [HL2640] (GTX639096) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX639096/GTX639096_45257_20250718_WB_multiple_B_25072423_528.webp)







