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Western Blot analysis of rat stomach cells using RASSF8 Polyclonal Antibody
Western Blot analysis of rat stomach cells using RASSF8 Polyclonal Antibody
Western Blot analysis of rat stomach cells using RASSF8 Polyclonal Antibody

RASSF8 Antibody

CSB-PA004748
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Rat
TargetRASSF8
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Overview

  • Supplier
    Cusabio
  • Product Name
    RASSF8 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID11228
  • Target name
    RASSF8
  • Target description
    Ras association domain family member 8
  • Target synonyms
    C12orf2; carcinoma-associated protein HOJ-1; HOJ1; Ras association (RalGDS/AF-6) domain family (N-terminal) member 8; ras association domain-containing protein 8
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8NHQ8
  • Protein Name
    Ras association domain-containing protein 8
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RASSF8 using anti-RASSF8 antibody (A11627-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human placenta tissue lysates, Lane 4: human U251 whole cell lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RASSF8 antigen affinity purified polyclonal antibody (Catalog # A11627-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RASSF8 at approximately 48 kDa. The expected band size for RASSF8 is at 48 kDa.
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