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ProductsBack/Rat Anti-Human IL-13-BIOT
ELISA plate was coated with recombinant human IL-13 and purified/unlabeled Mouse IgG2bkappa. Recombinant protein and purified immunoglobulin were detected with serially diluted Rat Anti-Human IL-13-BIOT (SB Cat. No. 10126-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
ELISA plate was coated with recombinant human IL-13 and purified/unlabeled Mouse IgG2bkappa. Recombinant protein and purified immunoglobulin were detected with serially diluted Rat Anti-Human IL-13-BIOT (SB Cat. No. 10126-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
ELISA plate was coated with recombinant human IL-13 and purified/unlabeled Mouse IgG2bkappa. Recombinant protein and purified immunoglobulin were detected with serially diluted Rat Anti-Human IL-13-BIOT (SB Cat. No. 10126-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).

Rat Anti-Human IL-13-BIOT

10126-08
SouthernBiotech
ApplicationsELISA
Product group Antibodies
ReactivityHuman
TargetIL13
0.5 mg
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Overview

  • Supplier
    SouthernBiotech
  • Product Name
    Rat Anti-Human IL-13-BIOT
  • Delivery Days Customer
    7
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    JES10-2E10
  • Conjugate
    Biotin
  • Gene ID3596
  • Target name
    IL13
  • Target description
    interleukin 13
  • Target synonyms
    IL-13, P600, interleukin-13
  • Host
    Rat
  • Isotype
    IgG1
  • Protein IDP35225
  • Protein Name
    Interleukin-13
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IL13 using anti-IL13 antibody (A00077). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL13 antigen affinity purified polyclonal antibody (Catalog # A00077) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL13 at approximately 20KD. The expected band size for IL13 is at 16KD.
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