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ProductsBack/Rat Anti-Mouse CD11b-CY5
BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD11b-CY5 (SB Cat. No. 1560-15) and Rat Anti-Mouse Ly-6G/Ly-6C-FITC (SB Cat. No. 1900-02).
BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD11b-CY5 (SB Cat. No. 1560-15) and Rat Anti-Mouse Ly-6G/Ly-6C-FITC (SB Cat. No. 1900-02).
BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD11b-CY5 (SB Cat. No. 1560-15) and Rat Anti-Mouse Ly-6G/Ly-6C-FITC (SB Cat. No. 1900-02).

Rat Anti-Mouse CD11b-CY5

1560-15
SouthernBiotech
ApplicationsFlow Cytometry, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
TargetItgam
Price on request
0.1 mg
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Overview

  • Supplier
    SouthernBiotech
  • Product Name
    Rat Anti-Mouse CD11b-CY5
  • Delivery Days Customer
    7
  • Applications
    Flow Cytometry, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3A33
  • Conjugate
    Cy5
  • Gene ID16409
  • Target name
    Itgam
  • Target description
    integrin alpha M
  • Target synonyms
    CD11 antigen-like family member B; Cd11b; CD11B (p170); CD11b/CD18; cell surface glycoprotein MAC-1 alpha subunit; cell surface glycoprotein MAC-1 subunit alpha; complement receptor type 3; CR; CR3; CR-3 alpha chain; CR3A; F730045J24Rik; integrin alpha-M; leukocyte adhesion receptor MO1; Ly-4; Ly-40; Mac-; MAC1; Mac-1; Mac-1 alpha; Mac-1a; macrophage antigen alpha
  • Host
    Rat
  • Isotype
    IgG2a
  • Protein IDP05555
  • Protein Name
    Integrin alpha-M
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD11B/Integrin Alpha M/Itgam using anti-CD11B/Integrin Alpha M/Itgam antibody (A00144-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse ANA-1 whole cell lysates, Lane 2: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD11B/Integrin Alpha M/Itgam antigen affinity purified polyclonal antibody (Catalog # A00144-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD11B/Integrin Alpha M/Itgam at approximately 170 kDa. The expected band size for CD11B/Integrin Alpha M/Itgam is at 170 kDa.
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