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CD11b antibody detects CD11b protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. CD11b stained by CD11b antibody (GTX134493) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
CD11b antibody detects CD11b protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. CD11b stained by CD11b antibody (GTX134493) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
CD11b antibody detects CD11b protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. CD11b stained by CD11b antibody (GTX134493) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

CD11b antibody

GTX134493
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityMouse, Rat
TargetItgam
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Overview

  • Supplier
    GeneTex
  • Product Name
    CD11b antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.17 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID16409
  • Target name
    Itgam
  • Target description
    integrin alpha M
  • Target synonyms
    CD11b/CD18, CR3, CR3A, Cd11b, F730045J24Rik, Ly-40, MAC1, Mac-1, Mac-1a, integrin alpha-M, CD11 antigen-like family member B, CD11B (p170), CR-3 alpha chain, Mac-1 alpha, cell surface glycoprotein MAC-1 alpha subunit, cell surface glycoprotein MAC-1 subunit alpha, complement receptor type 3, leukocyte adhesion receptor MO1, macrophage antigen alpha
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CD11B/Integrin Alpha M/Itgam using anti-CD11B/Integrin Alpha M/Itgam antibody (A00144-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse ANA-1 whole cell lysates, Lane 2: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD11B/Integrin Alpha M/Itgam antigen affinity purified polyclonal antibody (Catalog # A00144-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD11B/Integrin Alpha M/Itgam at approximately 170 kDa. The expected band size for CD11B/Integrin Alpha M/Itgam is at 170 kDa.
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