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ProductsBack/Rat Anti-Mouse Ly-6G/Ly-6C-LE/AF

Rat Anti-Mouse Ly-6G/Ly-6C-LE/AF

1900-14
SouthernBiotech
ApplicationsFlow Cytometry, ImmunoPrecipitation, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, Other Application
Product group Antibodies
ReactivityMouse
TargetLy6c1
0.5 mg
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Overview

  • Supplier
    SouthernBiotech
  • Product Name
    Rat Anti-Mouse Ly-6G/Ly-6C-LE/AF
  • Delivery Days Customer
    7
  • Applications
    Flow Cytometry, ImmunoPrecipitation, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, Other Application
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    RB6-8C5
  • Gene ID17067
  • Target name
    Ly6c1
  • Target description
    lymphocyte antigen 6 family member C1
  • Target synonyms
    Ly-6C, Ly-6C1, Ly6c, lymphocyte antigen 6C1, Lymphocyte antigen Ly-6C, lymphocyte antigen 6 complex, locus C, lymphocyte antigen 6 complex, locus C1
  • Host
    Rat
  • Isotype
    IgG2b
  • Protein IDP0CW02
  • Protein Name
    Lymphocyte antigen 6C1
  • Reactivity
    Mouse
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LY6C using anti-LY6C antibody (A33313). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse RAW264.7 whole cell lysates, Lane 2: mouse ANA-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LY6C antigen affinity purified polyclonal antibody (Catalog # A33313) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LY6C at approximately 14KD. The expected band size for LY6C is at 14KD.
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