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ProductsBack/Rat MIP-1Alpha/CCL3 ELISA Kit EZ-Set(tm) (DIY Antibody Pairs)
Rat MIP-1alpha/CCL3 EZ Set ELISA Kit standard curve
Rat MIP-1alpha/CCL3 EZ Set ELISA Kit standard curve
Rat MIP-1alpha/CCL3 EZ Set ELISA Kit standard curve

Rat MIP-1Alpha/CCL3 ELISA Kit EZ-Set(tm) (DIY Antibody Pairs)

EZ1219
Boster Bio
ApplicationsELISA
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetCcl3
5 plates
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Rat MIP-1Alpha/CCL3 EZ-Set ELISA Kit (DIY Antibody Pairs)
  • Delivery Days Customer
    9
  • Applications
    ELISA
  • Assay Detection Range
    7.8 pg/ml - 500 pg/ml
  • Certification
    Research Use Only
  • Gene ID25542
  • Target name
    Ccl3
  • Target description
    C-C motif chemokine ligand 3
  • Target synonyms
    C-C motif chemokine 3; chemokine (C-C motif) ligand 3; Macrophage inflammatory protein 1 alpha (Small inducible cytokine A3); macrophage inflammatory protein 1-alpha; MIP-1a; MIP-1-alpha; Scya3; small-inducible cytokine A3
  • Protein IDP50229
  • Protein Name
    C-C motif chemokine 3
  • Scientific Description
    Rat MIP-1Alpha/CCL3 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Rat Ccl3 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CCL3 using anti-CCL3 antibody (PB9022). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant Rat CCL3 Protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCL3 antigen affinity purified polyclonal antibody (Catalog # PB9022) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCL3 at approximately 33KD. The expected band size for CCL3 is at 33KD.
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