WB analysis of various samples using GTX00682 Rb (phospho Thr821) antibody [24A7]. The cell extract were immuno-precipitated with anti-Myc antibody and then analyzed by Western Blot with GTX00682 Rb (phospho Thr821) antibody [24A7]. Lane 1 : H1299 overexpressing Myc-tagged wild-type Rb Lane 2 : H1299 overexpressing Myc-tagged Rb (S795A) Lane 3 : H1299 overexpressing Myc-tagged Rb (T821A).
WB analysis of various samples using GTX00682 Rb (phospho Thr821) antibody [24A7]. The cell extract were immuno-precipitated with anti-Myc antibody and then analyzed by Western Blot with GTX00682 Rb (phospho Thr821) antibody [24A7]. Lane 1 : H1299 overexpressing Myc-tagged wild-type Rb Lane 2 : H1299 overexpressing Myc-tagged Rb (S795A) Lane 3 : H1299 overexpressing Myc-tagged Rb (T821A).
Rb (phospho Thr821) antibody [24A7]
GTX00682
Overview
- SupplierGeneTex
- Product NameRb (phospho Thr821) antibody [24A7] - Orthogonal Validated
- Delivery Days Customer9
- Application Supplier NoteWB: ~1 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID24A7
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID5925
- Target nameRB1
- Target descriptionRB transcriptional corepressor 1
- Target synonymsexon 17 tumor GOS561 substitution mutation causes premature stop; GOS563 exon 17 substitution mutation causes premature stop; OSRC; p105-Rb; p110-RB1; pp110; PPP1R130; pRb; prepro-retinoblastoma-associated protein; protein phosphatase 1, regulatory subunit 130; RB; retinoblastoma 1; retinoblastoma suspectibility protein; retinoblastoma-associated protein
- HostMouse
- IsotypeIgG1
- Protein IDP06400
- Protein NameRetinoblastoma-associated protein
- Scientific DescriptionThe protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203