Immunohistochemical staining of mouse tissue using anti-pRb2/p130 antiserum.? The staining shows the location of pRb2/p130 in developing mouse tissue.? Other detection systems should yield similar results. Sections were cut at 5-7 microm, mounted on glass and dried overnight at 37oC. ?All sections were deparaffinized in xylene, rehydrated through a graded alcohol series and washed in phosphate-buffered saline (PBS). ?PBS was used for all subsequent washes and for antiserum dilution. ?Tissue sections were quenched sequentially in 0.5% hydrogen peroxide and blocked with diluted 10% normal goat anti-rabbit serum. ?Slides were incubated at 20o C for 1 h with rabbit anti-pRb2/p130 (1:500) dilution, washed, and then reacted with diluted goat anti-rabbit biotinylated antibody for 30 min. ?Slides were then reacted with streptavidin-peroxidase conjugate for 30 min at 20o C.? Diaminobenzidine was used as the final chromogen. ?Negative controls for each tissue section were prepared by substituting the primary antiserum with pre-immune serum.
Immunohistochemical staining of mouse tissue using anti-pRb2/p130 antiserum.? The staining shows the location of pRb2/p130 in developing mouse tissue.? Other detection systems should yield similar results. Sections were cut at 5-7 microm, mounted on glass and dried overnight at 37oC. ?All sections were deparaffinized in xylene, rehydrated through a graded alcohol series and washed in phosphate-buffered saline (PBS). ?PBS was used for all subsequent washes and for antiserum dilution. ?Tissue sections were quenched sequentially in 0.5% hydrogen peroxide and blocked with diluted 10% normal goat anti-rabbit serum. ?Slides were incubated at 20o C for 1 h with rabbit anti-pRb2/p130 (1:500) dilution, washed, and then reacted with diluted goat anti-rabbit biotinylated antibody for 30 min. ?Slides were then reacted with streptavidin-peroxidase conjugate for 30 min at 20o C.? Diaminobenzidine was used as the final chromogen. ?Negative controls for each tissue section were prepared by substituting the primary antiserum with pre-immune serum.
Rb2 / p130 antibody
GTX26545
ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityMouse
TargetRBL2
Overview
- SupplierGeneTex
- Product NameRb2 / p130 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:2000. IHC-P: 1:200-1:1000. IP: 1:100. ELISA: 1:5000-1:20000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration85 mg/ml
- ConjugateUnconjugated
- Gene ID5934
- Target nameRBL2
- Target descriptionRB transcriptional corepressor like 2
- Target synonyms130 kDa retinoblastoma-associated protein; P130; PRB2; Rb2; RBR-2; retinoblastoma-like 2; retinoblastoma-like protein 2; retinoblastoma-related protein 2
- HostRabbit
- IsotypeIgG
- Protein IDQ08999
- Protein NameRetinoblastoma-like protein 2
- Scientific DescriptionThe product of the Retinoblastoma related human gene RB2 (p130) is highly homologous to the product of the Rb tumor suppressor gene, Rb (p105), especially in the functional pocket region of the protein. Certain carcinoma derived cell lines show low levels of Rbs expression, possibly due to gene rearrangement. In other experiments, a drastic reduction in proliferation is observed upon introduction of active Rb2.
- ReactivityMouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203