Western Blot of Rabbit Anti-Rb2 p130 Antibody. Lane 1: HEK 293 pcDNA3. Lane 2: HEK 293 pcDNA3-Rb2wt. Lane 3: HEK 293 pcDNA3-Rb2-PM19. Load: 30 μg per lane. Primary antibody: Anti-Rb2 antibody at 1:250 for overnight at 4oC. Secondary antibody: IRDye800? rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4oC. Predicted/Observed size: 130 kDa for p130/Rb2.
Western Blot of Rabbit Anti-Rb2 p130 Antibody. Lane 1: HEK 293 pcDNA3. Lane 2: HEK 293 pcDNA3-Rb2wt. Lane 3: HEK 293 pcDNA3-Rb2-PM19. Load: 30 μg per lane. Primary antibody: Anti-Rb2 antibody at 1:250 for overnight at 4oC. Secondary antibody: IRDye800? rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4oC. Predicted/Observed size: 130 kDa for p130/Rb2.
Rb2 / p130 antibody
GTX26545
ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetRBL2
Overview
- SupplierGeneTex
- Product NameRb2 / p130 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:2000. IHC-P: 1:200-1:1000. IP: 1:100. ELISA: 1:5000-1:20000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration85 mg/ml
- ConjugateUnconjugated
- Gene ID5934
- Target nameRBL2
- Target descriptionRB transcriptional corepressor like 2
- Target synonymsBRUWAG, P130, Rb2, retinoblastoma-like protein 2, 130 kDa retinoblastoma-associated protein, PRB2, RBR-2, retinoblastoma-like 2, retinoblastoma-related protein 2
- HostRabbit
- IsotypeIgG
- Protein IDQ08999
- Protein NameRetinoblastoma-like protein 2
- Scientific DescriptionThe product of the Retinoblastoma related human gene RB2 (p130) is highly homologous to the product of the Rb tumor suppressor gene, Rb (p105), especially in the functional pocket region of the protein. Certain carcinoma derived cell lines show low levels of Rbs expression, possibly due to gene rearrangement. In other experiments, a drastic reduction in proliferation is observed upon introduction of active Rb2.
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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