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ProductsBack/Rb2 / p130 antibody [KAB40]

Rb2 / p130 antibody [KAB40]

GTX10576
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetRBL2
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Overview

  • Supplier
    GeneTex
  • Product Name
    Rb2 / p130 antibody [KAB40]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: Use at a dilution of 1/1000. Detects a band of approximately 130 kDa. Optimal dilutions/concentrations should be determined by the end user.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    KAB40
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5934
  • Target name
    RBL2
  • Target description
    RB transcriptional corepressor like 2
  • Target synonyms
    130 kDa retinoblastoma-associated protein; P130; PRB2; Rb2; RBR-2; retinoblastoma-like 2; retinoblastoma-like protein 2; retinoblastoma-related protein 2
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ08999
  • Protein Name
    Retinoblastoma-like protein 2
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Immunohistochemical staining of mouse tissue using anti-pRb2/p130 antiserum.? The staining shows the location of pRb2/p130 in developing mouse tissue.? Other detection systems should yield similar results. Sections were cut at 5-7 microm, mounted on glass and dried overnight at 37oC. ?All sections were deparaffinized in xylene, rehydrated through a graded alcohol series and washed in phosphate-buffered saline (PBS). ?PBS was used for all subsequent washes and for antiserum dilution. ?Tissue sections were quenched sequentially in 0.5% hydrogen peroxide and blocked with diluted 10% normal goat anti-rabbit serum. ?Slides were incubated at 20o C for 1 h with rabbit anti-pRb2/p130 (1:500) dilution, washed, and then reacted with diluted goat anti-rabbit biotinylated antibody for 30 min. ?Slides were then reacted with streptavidin-peroxidase conjugate for 30 min at 20o C.? Diaminobenzidine was used as the final chromogen. ?Negative controls for each tissue section were prepared by substituting the primary antiserum with pre-immune serum.
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