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Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA853432ESR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA853432ESR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA853432ESR1HU at dilution of 1:100

RBM17 Antibody

CSB-PA853432ESR1HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRBM17
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Overview

  • Supplier
    Cusabio
  • Product Name
    RBM17 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID84991
  • Target name
    RBM17
  • Target description
    RNA binding motif protein 17
  • Target synonyms
    SPF45, splicing factor 45, 45 kDa-splicing factor, splicing factor 45kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96I25
  • Protein Name
    Splicing factor 45
  • Scientific Description
    Splice factor that binds to the single-stranded 3AG at the exon/intron border and promotes its utilization in the second catalytic step. Involved in the regulation of alternative splicing and the utilization of cryptic splice sites. Promotes the utilization of a cryptic splice site created by the beta-110 mutation in the HBB gene. The resulting frameshift leads to sickle cell anemia.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RBM17 using anti-RBM17 antibody (A08621-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse RAW264.7 whole cell lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBM17 antigen affinity purified polyclonal antibody (Catalog # A08621-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RBM17 at approximately 45 kDa. The expected band size for RBM17 is at 45 kDa.
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