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ProductsBack/RBM17 antibody [N3C3]
Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE GTX120047 diluted at 1:500
Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE GTX120047 diluted at 1:500
Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE GTX120047 diluted at 1:500

RBM17 antibody [N3C3]

GTX120047
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetRBM17
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Overview

  • Supplier
    GeneTex
  • Product Name
    RBM17 antibody [N3C3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID84991
  • Target name
    RBM17
  • Target description
    RNA binding motif protein 17
  • Target synonyms
    SPF45, splicing factor 45, 45 kDa-splicing factor, splicing factor 45kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96I25
  • Protein Name
    Splicing factor 45
  • Scientific Description
    This gene encodes an RNA binding protein. The encoded protein is part of the spliceosome complex and functions in the second catalytic step of mRNA splicing. Alternatively spliced transcript variants have been described. Related pseudogenes exist on chromosomes 9 and 15. [provided by RefSeq]
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RBM17 using anti-RBM17 antibody (A08621-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse RAW264.7 whole cell lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBM17 antigen affinity purified polyclonal antibody (Catalog # A08621-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RBM17 at approximately 45 kDa. The expected band size for RBM17 is at 45 kDa.
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