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Immunohistochemistry of paraffin-embedded human skin tissue using CSB-PA019420HA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human skin tissue using CSB-PA019420HA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human skin tissue using CSB-PA019420HA01HU at dilution of 1:100

RBM3 Antibody

CSB-PA019420HA01HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRBM3
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Overview

  • Supplier
    Cusabio
  • Product Name
    RBM3 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5935
  • Target name
    RBM3
  • Target description
    RNA binding motif protein 3
  • Target synonyms
    IS1-RNPL, RNPL, RNA-binding protein 3, RNA binding motif (RNP1, RRM) protein 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP98179
  • Protein Name
    RNA-binding protein 3
  • Scientific Description
    Cold-inducible mRNA binding protein that enhances global protein synthesis at both physiological and mild hypothermic temperatures. Reduces the relative abundance of microRNAs, when overexpressed. Enhances phosphorylation of translation initiation factors and active polysome formation (By similarity).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RBM3 using anti-RBM3 antibody (M03104). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBM3 antigen affinity purified monoclonal antibody (Catalog # M03104) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RBM3 at approximately 17 kDa. The expected band size for RBM3 is at 17 kDa.
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