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Western Blot analysis of K562 cells using CRBP II Polyclonal Antibody.
Western Blot analysis of K562 cells using CRBP II Polyclonal Antibody.
Western Blot analysis of K562 cells using CRBP II Polyclonal Antibody.

RBP2 Antibody

CSB-PA005623
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetRBP2
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Overview

  • Supplier
    Cusabio
  • Product Name
    RBP2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5948
  • Target name
    RBP2
  • Target description
    retinol binding protein 2
  • Target synonyms
    CRABP-II, CRBP2, CRBPII, RBPC2, retinol-binding protein 2, CRBP-II, cellular retinol-binding protein II, retinol binding protein 2, cellular
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP50120
  • Protein Name
    Retinol-binding protein 2
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RBP2 using anti-RBP2 antibody (A06064-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: rat small intestines tissue lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse small intestines tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBP2 antigen affinity purified polyclonal antibody (Catalog # A06064-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RBP2 at approximately 16 kDa. The expected band size for RBP2 is at 16 kDa.
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