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WB analysis of various sample lysates using GTX33462 RBP2 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX33462 RBP2 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX33462 RBP2 antibody. Dilution : 1:1000 Loading : 25microg per lane

RBP2 antibody

GTX33462
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetRBP2
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Overview

  • Supplier
    GeneTex
  • Product Name
    RBP2 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. ICC/IF: 1:50 - 1:100. IHC-P: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5948
  • Target name
    RBP2
  • Target description
    retinol binding protein 2
  • Target synonyms
    cellular retinol-binding protein II; CRABP-II; CRBP2; CRBPII; CRBP-II; RBPC2; retinol binding protein 2, cellular; retinol-binding protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP50120
  • Protein Name
    Retinol-binding protein 2
  • Scientific Description
    This gene encodes an abundant protein present in the small intestinal epithelium. It is thought to participate in the uptake and/or intracellular metabolism of vitamin A. Vitamin A is a fat-soluble vitamin necessary for growth, reproduction, differentiation of epithelial tissues, and vision. This protein may also modulate the supply of retinoic acid to the nuclei of endometrial cells during the menstrual cycle. [provided by RefSeq, Aug 2015]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RBP2 using anti-RBP2 antibody (A06064-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: rat small intestines tissue lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse small intestines tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBP2 antigen affinity purified polyclonal antibody (Catalog # A06064-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RBP2 at approximately 16 kDa. The expected band size for RBP2 is at 16 kDa.
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