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ProductsBack/RBP3 / IRBP Antibody

RBP3 / IRBP Antibody

LS-C831436
LifeSpan BioSciences
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetRBP3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    RBP3 / IRBP Antibody
  • Delivery Days Customer
    23
  • Applications
    ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:50 - 1:100)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5949
  • Target name
    RBP3
  • Target description
    retinol binding protein 3
  • Target synonyms
    D10S64, D10S65, D10S66, IRBP, RBPI, RP66, retinol-binding protein 3, interphotoreceptor retinoid-binding protein, interstitial retinol binding protein 3, retinol binding protein 3, interstitial
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IRBP/RBP3 using anti-IRBP/RBP3 antibody (A02913-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat eye tissue lysates, Lane 2: mouse eye tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRBP/RBP3 antigen affinity purified polyclonal antibody (Catalog # A02913-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IRBP/RBP3 at approximately 135 kDa. The expected band size for IRBP/RBP3 is at 135 kDa.
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