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RBPJK Antibody

HY-P80305
MedChem Express
TargetRBPJ
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    RBPJK Antibody
  • Delivery Days Customer
    10
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID3516
  • Target name
    RBPJ
  • Target description
    recombination signal binding protein for immunoglobulin kappa J region
  • Target synonyms
    AOS3; CBF1; CBF-1; csl; H-2K binding factor-2; IGKJRB; IGKJRB1; immunoglobulin kappa J region recombination signal binding protein 1; KBF2; RBP-J; RBP-J kappa; RBPJK; RBP-JK; RBPSUH; recombining binding protein suppressor of hairless; renal carcinoma antigen NY-REN-30; SUH; suppressor of hairless homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    RBPJK Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 56 kDa, targeting to RBPJK. It can be used for WB assays with tag free, in the background of Human.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RBPJ using anti-RBPJ antibody (A00767-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat NRK whole cell lysates, Lane 3: mouse NIH3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBPJ antigen affinity purified polyclonal antibody (Catalog # A00767-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RBPJ at approximately 60KD. The expected band size for RBPJ is at 56KD.
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