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Lane 1: mouse embryo lysates Lane 2: human colon carcinoma lysates probed with Anti RBPJK/RBP-J Polyclonal Antibody, Unconjugated (bs-2966R) at 1:200 in 4C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37C. Predicted band 55kD. Observed band size: 55kD
Lane 1: mouse embryo lysates Lane 2: human colon carcinoma lysates probed with Anti RBPJK/RBP-J Polyclonal Antibody, Unconjugated (bs-2966R) at 1:200 in 4C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37C. Predicted band 55kD. Observed band size: 55kD
Lane 1: mouse embryo lysates Lane 2: human colon carcinoma lysates probed with Anti RBPJK/RBP-J Polyclonal Antibody, Unconjugated (bs-2966R) at 1:200 in 4C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37C. Predicted band 55kD. Observed band size: 55kD

RBPJK Polyclonal Antibody

BS-2966R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetRBPJ
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    RBPJK Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID3516
  • Target name
    RBPJ
  • Target description
    recombination signal binding protein for immunoglobulin kappa J region
  • Target synonyms
    AOS3; CBF1; CBF-1; csl; H-2K binding factor-2; IGKJRB; IGKJRB1; immunoglobulin kappa J region recombination signal binding protein 1; KBF2; RBP-J; RBP-J kappa; RBPJK; RBP-JK; RBPSUH; recombining binding protein suppressor of hairless; renal carcinoma antigen NY-REN-30; SUH; suppressor of hairless homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ06330
  • Protein Name
    Recombining binding protein suppressor of hairless
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RBPJ using anti-RBPJ antibody (A00767-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat NRK whole cell lysates, Lane 3: mouse NIH3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBPJ antigen affinity purified polyclonal antibody (Catalog # A00767-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RBPJ at approximately 60KD. The expected band size for RBPJ is at 56KD.
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