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ProductsBack/RBPMS / Hermes Antibody

RBPMS / Hermes Antibody

LS-C750367
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetRBPMS
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    RBPMS / Hermes Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 15kDa/17kDa/21kDa/22kDa/24kDa, while the observed MW by Western blot was 22kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:200 - 1:2000) The predicted MW is 15kDa/17kDa/21kDa/22kDa/24kDa, while the observed MW by Western blot was 22kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID11030
  • Target name
    RBPMS
  • Target description
    RNA binding protein, mRNA processing factor
  • Target synonyms
    HERMES, RNA-binding protein with multiple splicing, RNA binding protein with multiple splicing, heart and RRM expressed sequence
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RBPMS using anti-RBPMS antibody (A07130-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse NIH/3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBPMS antigen affinity purified polyclonal antibody (Catalog # A07130-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RBPMS at approximately 22KD. The expected band size for RBPMS is at 22KD.
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