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Western blot analysis of extracts of various cell lines using RDX Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of various cell lines using RDX Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of various cell lines using RDX Polyclonal Antibody at dilution of 1:1000.

RDX Polyclonal Antibody

E-AB-65106
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    RDX Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    Radixin is a cytoskeletal protein that may be important in linking actin to the plasma membrane. It is highly similar in sequence to both ezrin and moesin. The radixin gene has been localized by fluorescence in situ hybridization to 11q23. A truncated version representing a pseudogene (RDXP2) was assigned to Xp21.3. Another pseudogene that seemed to lack introns (RDXP1) was mapped to 11p by Southern and PCR analyses. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RDX using anti-RDX antibody (A01926-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human U2OS whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human A431 whole cell lysates, Lane 6: human PC-3 whole cell lysates, Lane 7: human HepG2 whole cell lysates, Lane 8: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RDX antigen affinity purified polyclonal antibody (Catalog # A01926-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RDX at approximately 88KD. The expected band size for RDX is at 69KD.
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ReactivityHuman, Mouse, Rat
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Product group Antibodies
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Paraformaldehyde-fixed, paraffin embedded Rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Radixin Polyclonal Antibody, Unconjugated (bs-3808R) at 1:200 overnight at 4°C, DAB staining.
Product group Antibodies
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Immunohistochemical staining of human adrenal gland shows strong membranous and cytoplasmic positivity in glandular cells.
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