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ProductsBack/Relaxin 1 (RLN1) Mouse Monoclonal Antibody [Clone ID: 6F1willi]

Relaxin 1 (RLN1) Mouse Monoclonal Antibody [Clone ID: 6F1willi]

AM02203PU-N
OriGene
ApplicationsFlow Cytometry, ELISA
Product group Antibodies
ReactivityHuman
TargetRLN1
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    Relaxin 1 (RLN1) Mouse Monoclonal Antibody [Clone ID: 6F1willi]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    6F1willi
  • Gene ID6013
  • Target name
    RLN1
  • Target description
    relaxin 1
  • Target synonyms
    H1, H1RLX, RLXH1, bA12D24.3.1, bA12D24.3.2, prorelaxin H1, preprorelaxin H1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP04808
  • Protein Name
    Prorelaxin H1
  • Scientific Description
    Relaxin 1 (RLN1) mouse monoclonal antibody, clone 6F1willi, Purified
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of Relaxin 1 using anti-Relaxin 1 antibody (A08367-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat PC-12 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Relaxin 1 antigen affinity purified polyclonal antibody (Catalog # A08367-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Relaxin 1 at approximately 21KD. The expected band size for Relaxin 1 is at 21KD.
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