![Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with RelB antibody [HL2222] (GTX638267) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with RelB antibody [HL2222] (GTX638267) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638267/GTX638267_T-44956_20230217_WB_M_23022022_620.webp)
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with RelB antibody [HL2222] (GTX638267) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
RelB antibody [HL2222]
GTX638267
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetRELB
Overview
- SupplierGeneTex
- Product NameRelB antibody [HL2222]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2222
- Concentration2 mg/ml
- ConjugateUnconjugated
- Gene ID5971
- Target nameRELB
- Target descriptionRELB proto-oncogene, NF-kB subunit
- Target synonymsI-REL, IMD53, IREL, REL-B, transcription factor RelB, v-rel avian reticuloendotheliosis viral oncogene homolog B (nuclear factor of kappa light polypeptide gene enhancer in B-cells 3), v-rel reticuloendotheliosis viral oncogene homolog B, nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
- HostRabbit
- IsotypeIgG
- Protein IDQ01201
- Protein NameTranscription factor RelB
- Scientific DescriptionEnables mRNA N6-methyladenosine dioxygenase activity. Involved in RNA metabolic process; mRNA export from nucleus; and response to hypoxia. Located in Golgi apparatus; cytosol; and nuclear speck. [provided by Alliance of Genome Resources, Apr 2022]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RelB antibody [HL2222] (GTX638267) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RelB antibody [HL2222] (GTX638267) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638267/GTX638267_T-44956_20230217_WB_R_23022022_600.webp)
![RelB antibody [HL2222] detects RelB protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat colon. RelB stained by RelB antibody [HL2222] (GTX638267) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min RelB antibody [HL2222] detects RelB protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat colon. RelB stained by RelB antibody [HL2222] (GTX638267) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX638267/GTX638267_T-44956_20230325_IHC-P_R_23032819_282.webp)
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RelB antibody [HL2222] (GTX638267) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RelB antibody [HL2222] (GTX638267) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638267/GTX638267_45026_20230428_WB_TPM_watermark_23050223_563.webp)
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RelB antibody [HL2222] (GTX638267) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RelB antibody [HL2222] (GTX638267) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638267/GTX638267_45026_20230707_WB_B_23071223_371.webp)
![Immunoprecipitation of RelB protein from Raji whole cell extract using 5 μg of RelB antibody [HL2222] (GTX638267). Western blot analysis was performed using RelB antibody [HL2222] (GTX638267). EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01). Immunoprecipitation of RelB protein from Raji whole cell extract using 5 μg of RelB antibody [HL2222] (GTX638267). Western blot analysis was performed using RelB antibody [HL2222] (GTX638267). EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01).](https://www.genetex.com/upload/website/prouct_img/normal/GTX638267/GTX638267_T-44956_20240419_IP_24042400_827.webp)




