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ProductsBack/RFC3 Antibody (aa80-110)

RFC3 Antibody (aa80-110)

LS-C287111
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetRFC3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    RFC3 Antibody (aa80-110)
  • Delivery Days Customer
    23
  • Application Supplier Note
    IHC: Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:500 - 1:2000), WB (1:2500 - 1:10000) IHC: Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5983
  • Target name
    RFC3
  • Target description
    replication factor C subunit 3
  • Target synonyms
    RFC38, replication factor C subunit 3, A1 38 kDa subunit, RF-C 38 kDa subunit, RFC, 38 kD subunit, activator 1 38 kDa subunit, activator 1 subunit 3, replication factor C (activator 1) 3, 38kDa, replication factor C 38 kDa subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RFC3 using anti-RFC3 antibody (A06442-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Daudi whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RFC3 antigen affinity purified polyclonal antibody (Catalog # A06442-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RFC3 at approximately 40 kDa. The expected band size for RFC3 is at 41 kDa.
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