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Western Blot analysis of HepG2 cells using RFC3 Polyclonal Antibody
Western Blot analysis of HepG2 cells using RFC3 Polyclonal Antibody
Western Blot analysis of HepG2 cells using RFC3 Polyclonal Antibody

RFC3 Antibody

CSB-PA003964
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetRFC3
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Overview

  • Supplier
    Cusabio
  • Product Name
    RFC3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5983
  • Target name
    RFC3
  • Target description
    replication factor C subunit 3
  • Target synonyms
    A1 38 kDa subunit; activator 1 38 kDa subunit; activator 1 subunit 3; replication factor C (activator 1) 3, 38kDa; replication factor C 38 kDa subunit; replication factor C subunit 3; RF-C 38 kDa subunit; RFC, 38 kD subunit; RFC38
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP40938
  • Protein Name
    Replication factor C subunit 3
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RFC3 using anti-RFC3 antibody (A06442-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Daudi whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RFC3 antigen affinity purified polyclonal antibody (Catalog # A06442-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RFC3 at approximately 40 kDa. The expected band size for RFC3 is at 41 kDa.
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