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Western Blot analysis of 3T3 cells using Raftlin Polyclonal Antibody
Western Blot analysis of 3T3 cells using Raftlin Polyclonal Antibody
Western Blot analysis of 3T3 cells using Raftlin Polyclonal Antibody

RFTN1 Antibody

CSB-PA008331
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetRFTN1
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Overview

  • Supplier
    Cusabio
  • Product Name
    RFTN1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23180
  • Target name
    RFTN1
  • Target description
    raftlin, lipid raft linker 1
  • Target synonyms
    MIG2, PIB10, PIG9, RAFTLIN, raftlin, cell migration-inducing gene 2 protein, proliferation-inducing protein 10, proliferation-inducing protein 9, raft-linking protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14699
  • Protein Name
    Raftlin
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RFTN1 using anti-RFTN1 antibody (A11324-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RFTN1 antigen affinity purified polyclonal antibody (Catalog # A11324-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RFTN1 at approximately 70 kDa. The expected band size for RFTN1 is at 63 kDa.
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