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ProductsBack/RFTN1 / Raftlin Antibody

RFTN1 / Raftlin Antibody

LS-C497139
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetRFTN1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    RFTN1 / Raftlin Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 63kDa, while the observed MW by Western blot was 70kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 63kDa, while the observed MW by Western blot was 70kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID23180
  • Target name
    RFTN1
  • Target description
    raftlin, lipid raft linker 1
  • Target synonyms
    MIG2, PIB10, PIG9, RAFTLIN, raftlin, cell migration-inducing gene 2 protein, proliferation-inducing protein 10, proliferation-inducing protein 9, raft-linking protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RFTN1 using anti-RFTN1 antibody (A11324-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RFTN1 antigen affinity purified polyclonal antibody (Catalog # A11324-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RFTN1 at approximately 70 kDa. The expected band size for RFTN1 is at 63 kDa.
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