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ProductsBack/RFXANK Antibody (clone OTI3F5)

RFXANK Antibody (clone OTI3F5)

LS-C173729
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetRFXANK
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    RFXANK Antibody (clone OTI3F5)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    Flo (1:100), IF (1:100), IHC, IHC-P (1:150), WB (1:2000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI3F5
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8625
  • Target name
    RFXANK
  • Target description
    regulatory factor X associated ankyrin containing protein
  • Target synonyms
    ANKRA1; ankyrin repeat family A protein 1; ankyrin repeat-containing regulatory factor X-associated protein; BLS; DNA-binding protein RFXANK; F14150_1; regulatory factor X subunit B; RFX-B; RFX-Bdelta4
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RFXANK using anti-RFXANK antibody (A07934). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat thymus tissue lysates, Lane 6: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RFXANK antigen affinity purified polyclonal antibody (Catalog # A07934) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RFXANK at approximately 34 kDa. The expected band size for RFXANK is at 28 kDa.
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Western Blot Positive WB detected in: 293T whole cell lysate, SH-SY5Y whole cell lysate All lanes: RFXANK antibody at 6.7ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 29, 25, 26 kDa Observed band size: 29 kDa
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