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RFXANK Polyclonal Antibody

CAC15371
Biomatik
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetRFXANK
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Overview

  • Supplier
    Biomatik
  • Product Name
    RFXANK Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8625
  • Target name
    RFXANK
  • Target description
    regulatory factor X associated ankyrin containing protein
  • Target synonyms
    ANKRA1, BLS, F14150_1, MHC2D2, RFX-B, DNA-binding protein RFXANK, RFX-Bdelta4, ankyrin repeat family A protein 1, ankyrin repeat-containing regulatory factor X-associated protein, regulatory factor X subunit B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO14593
  • Protein Name
    DNA-binding protein RFXANK
  • Scientific Description
    The RFXANK Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC, IF.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: 293T whole cell lysate, SH-SY5Y whole cell lysate All lanes: RFXANK antibody at 6.7ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 29, 25, 26 kDa Observed band size: 29 kDa
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Figure 1. Western blot analysis of RFXANK using anti-RFXANK antibody (A07934). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat thymus tissue lysates, Lane 6: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RFXANK antigen affinity purified polyclonal antibody (Catalog # A07934) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RFXANK at approximately 34 kDa. The expected band size for RFXANK is at 28 kDa.
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Immunohistochemical staining of human kidney shows strong cytoplasmic and nuclear positivity in cells in tubules and strong nuclear staining in cells in glomeruli.
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