More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/RHOT1 Antibody

RHOT1 Antibody

LS-C334329
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetRHOT1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    RHOT1 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 28kDa/66kDa/70kDa/71kDa/74kDa/75kDa/79kDa, while the observed MW by Western blot was 80kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:50 - 1:100), WB (1:500 - 1:2000) The predicted MW is 28kDa/66kDa/70kDa/71kDa/74kDa/75kDa/79kDa, while the observed MW by Western blot was 80kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.31 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID55288
  • Target name
    RHOT1
  • Target description
    ras homolog family member T1
  • Target synonyms
    ARHT1, MIRO-1, MIRO1, mitochondrial Rho GTPase 1, mitochondrial Rho (MIRO) GTPase 1, rac-GTP binding protein-like protein, ras homolog gene family, member T1
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

Go to our frequently asked questions

Related products

Anti-RHOT1 Antibody
Product group Antibodies
Antibodies.com
Anti-RHOT1 AntibodyA31083
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice
Immunohistochemical staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neuronal cells, as well as positivity in neuropil.
Product group Antibodies
Atlas Antibodies
Anti-RHOT1 AntibodyAMAB90854
ApplicationsImmunoHistoChemistry
ReactivityHuman
TargetRHOT1
  • SizePrice
Product group Antibodies
RayBiotech
Anti-RHOT1 (N-term) Antibody102-23243
ApplicationsWestern Blot
TargetRHOT1
  • SizePrice
Product group Antibodies
Biosynth
RHOT1 antibody70R-5954
  • SizePrice
IHC-P analysis of mouse brain tissue using GTX31938 RHOT1 antibody. Working concentration : 5 microg/ml
Product group Antibodies
GeneTex
RHOT1 antibodyGTX31938
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetRHOT1
  • SizePrice
Figure 1. Western blot analysis of MIRO1/RHOT1 using anti-MIRO1/RHOT1 antibody (A05928-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse heart tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MIRO1/RHOT1 antigen affinity purified polyclonal antibody (Catalog # A05928-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MIRO1/RHOT1 at approximately 71 kDa. The expected band size for MIRO1/RHOT1 is at 71 kDa.
Product group Antibodies
Boster Bio
Anti-MIRO1/RHOT1 Antibody Picoband(r)A05928-1-CARRIER-FREE
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetRHOT1
  • SizePrice