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ProductsBack/Ribonuclease Inhibitor antibody [4G4]
FACS analysis of HeLa cells using GTX83697 Ribonuclease Inhibitor antibody [4G4]. Red : Primary antibody Blue : Negative control antibody
FACS analysis of HeLa cells using GTX83697 Ribonuclease Inhibitor antibody [4G4]. Red : Primary antibody Blue : Negative control antibody
FACS analysis of HeLa cells using GTX83697 Ribonuclease Inhibitor antibody [4G4]. Red : Primary antibody Blue : Negative control antibody

Ribonuclease Inhibitor antibody [4G4]

GTX83697
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetRNH1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Ribonuclease Inhibitor antibody [4G4]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:2000. ICC/IF: 1:100. IHC-P: 1:150. FACS: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    4G4
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6050
  • Target name
    RNH1
  • Target description
    ribonuclease/angiogenin inhibitor 1
  • Target synonyms
    placental ribonuclease inhibitor; placental RNase inhibitor; RAI; ribonuclease inhibitor; RNH; testicular tissue protein Li 164
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP13489
  • Protein Name
    Ribonuclease inhibitor
  • Scientific Description
    Inhibitor of pancreatic RNase and angiogenin. May also function in the modulation of cellular activities.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RNH1 using anti-RNH1 antibody (PB9885). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Hela whole cell lysates, Lane 3: human SK-OV-3 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human U-87MG whole cell lysates, Lane 6: monkey COS-7 whole cell lysates, Lane 7: human SW620 whole cell lysates, Lane 8: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNH1 antigen affinity purified polyclonal antibody (Catalog # PB9885) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNH1 at approximately 50KD. The expected band size for RNH1 is at 50KD.
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