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ProductsBack/Ribonuclease Inhibitor (RNH1) Mouse Monoclonal Antibody [Clone ID: OTI1B7]
Figure A, Western blot analysis of overexpressed lysates (5ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human RNH1 plasmid (RC208360, hRNH1), mouse RNH1 plasmid (MR207287, mRNH1) using anti-RNH1 antibody TA501981 (1:10000@1mg/ml). Figure B, Western blot analysis of the same samples as Figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml).
Figure A, Western blot analysis of overexpressed lysates (5ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human RNH1 plasmid (RC208360, hRNH1), mouse RNH1 plasmid (MR207287, mRNH1) using anti-RNH1 antibody TA501981 (1:10000@1mg/ml). Figure B, Western blot analysis of the same samples as Figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml).
Figure A, Western blot analysis of overexpressed lysates (5ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human RNH1 plasmid (RC208360, hRNH1), mouse RNH1 plasmid (MR207287, mRNH1) using anti-RNH1 antibody TA501981 (1:10000@1mg/ml). Figure B, Western blot analysis of the same samples as Figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml).

Ribonuclease Inhibitor (RNH1) Mouse Monoclonal Antibody [Clone ID: OTI1B7]

CF501981
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRNH1
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    Ribonuclease Inhibitor (RNH1) Mouse Monoclonal Antibody [Clone ID: OTI1B7]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI1B7
  • Gene ID6050
  • Target name
    RNH1
  • Target description
    ribonuclease/angiogenin inhibitor 1
  • Target synonyms
    IIAE12, RAI, RNH, ribonuclease inhibitor, placental RNase inhibitor, placental ribonuclease inhibitor, testicular tissue protein Li 164
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP13489
  • Protein Name
    Ribonuclease inhibitor
  • Scientific Description
    Carrier-free (BSA/glycerol-free) RNH1 mouse monoclonal antibody, clone OTI1B7 (formerly 1B7)
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RNH1 using anti-RNH1 antibody (PB9885). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Hela whole cell lysates, Lane 3: human SK-OV-3 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human U-87MG whole cell lysates, Lane 6: monkey COS-7 whole cell lysates, Lane 7: human SW620 whole cell lysates, Lane 8: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNH1 antigen affinity purified polyclonal antibody (Catalog # PB9885) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNH1 at approximately 50KD. The expected band size for RNH1 is at 50KD.
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