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ProductsBack/Ribonuclease Inhibitor (RNH1) Mouse Monoclonal Antibody [Clone ID: OTI4G4]
Equivalent amounts of cell lysates (30 ug per lane) of wild-type HepG2 cells(WT) and RNH1-Knockdown HepG2 cells(KD) were separated by SDS-PAGE and immunoblotted with anti-RNH1 monoclonal antibody TA501874(1:2500).Then the blotted membrane was stripped and reprobed with anti-HSP90AA1 antibody as a loading control.
Equivalent amounts of cell lysates (30 ug per lane) of wild-type HepG2 cells(WT) and RNH1-Knockdown HepG2 cells(KD) were separated by SDS-PAGE and immunoblotted with anti-RNH1 monoclonal antibody TA501874(1:2500).Then the blotted membrane was stripped and reprobed with anti-HSP90AA1 antibody as a loading control.
Equivalent amounts of cell lysates (30 ug per lane) of wild-type HepG2 cells(WT) and RNH1-Knockdown HepG2 cells(KD) were separated by SDS-PAGE and immunoblotted with anti-RNH1 monoclonal antibody TA501874(1:2500).Then the blotted membrane was stripped and reprobed with anti-HSP90AA1 antibody as a loading control.

Ribonuclease Inhibitor (RNH1) Mouse Monoclonal Antibody [Clone ID: OTI4G4]

CF501874
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRNH1
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    Ribonuclease Inhibitor (RNH1) Mouse Monoclonal Antibody [Clone ID: OTI4G4]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI4G4
  • Gene ID6050
  • Target name
    RNH1
  • Target description
    ribonuclease/angiogenin inhibitor 1
  • Target synonyms
    IIAE12, RAI, RNH, ribonuclease inhibitor, placental RNase inhibitor, placental ribonuclease inhibitor, testicular tissue protein Li 164
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP13489
  • Protein Name
    Ribonuclease inhibitor
  • Scientific Description
    Carrier-free (BSA/glycerol-free) RNH1 mouse monoclonal antibody, clone OTI4G4 (formerly 4G4)
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of RNH1 using anti-RNH1 antibody (PB9885). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Hela whole cell lysates, Lane 3: human SK-OV-3 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human U-87MG whole cell lysates, Lane 6: monkey COS-7 whole cell lysates, Lane 7: human SW620 whole cell lysates, Lane 8: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNH1 antigen affinity purified polyclonal antibody (Catalog # PB9885) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNH1 at approximately 50KD. The expected band size for RNH1 is at 50KD.
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