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ProductsBack/Ribophorin I Recombinant Antbody Kit (KD-Validated)

Ribophorin I Recombinant Antbody Kit (KD-Validated)

BSM-90533R
Bioss Antibodies
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
TargetRPN1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Ribophorin I Recombinant Antbody Kit (KD-Validated)
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, Western Blot
  • Applications Supplier
    WB(1:300-5000), FCM(1:20-100)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID6184
  • Target name
    RPN1
  • Target description
    ribophorin I
  • Target synonyms
    dolichyl-diphosphooligosaccharide-protein glycosyltransferase 67 kDa subunit; dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1; oligosaccharyltransferase 1 homolog; oligosaccharyltransferase complex subunit (non-catalytic); OST1; RBPH1; ribophorin-1; RPN-I
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP04843
  • Protein Name
    Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Ribophorin I/RPN1 using anti-Ribophorin I/RPN1 antibody (A05063-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ribophorin I/RPN1 antigen affinity purified polyclonal antibody (Catalog # A05063-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ribophorin I/RPN1 at approximately 68 kDa. The expected band size for Ribophorin I/RPN1 is at 68 kDa.
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Immunohistochemical staining of human cerebral cortex, colon, liver and tonsil using Anti-RPN1 antibody HPA026828 (A) shows similar protein distribution across tissues to independent antibody HPA051520 (B).
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