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ProductsBack/Ribosome binding protein 1 antibody [GT5610]
Ribosome binding protein 1 antibody [GT5610] detects Ribosome binding protein 1 protein at endoplasmic reticulum by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ribosome binding protein 1 protein stained by Ribosome binding protein 1 antibody [GT5610] (GTX628998) diluted at 1:2000. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Ribosome binding protein 1 antibody [GT5610] detects Ribosome binding protein 1 protein at endoplasmic reticulum by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ribosome binding protein 1 protein stained by Ribosome binding protein 1 antibody [GT5610] (GTX628998) diluted at 1:2000. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Ribosome binding protein 1 antibody [GT5610] detects Ribosome binding protein 1 protein at endoplasmic reticulum by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ribosome binding protein 1 protein stained by Ribosome binding protein 1 antibody [GT5610] (GTX628998) diluted at 1:2000. Blue: Hoechst 33342 staining. Scale bar = 10 μm.

Ribosome binding protein 1 antibody [GT5610]

GTX628998
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetRRBP1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Ribosome binding protein 1 antibody [GT5610]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    GT5610
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6238
  • Target name
    RRBP1
  • Target description
    ribosome binding protein 1
  • Target synonyms
    ES/130, ES130, RRp, hES, p180, ribosome-binding protein 1, 180 kDa ribosome receptor homolog, ES/130-related protein, endoplasmic reticulum ribosome-binding protein 1, ribosomal receptor-binding protein 1, ribosome binding protein 1 homolog 180kDa (dog), ribosome receptor protein
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDQ9P2E9
  • Protein Name
    Ribosome-binding protein 1
  • Scientific Description
    Analysis of cDNA clones indicates that ribosome binding protein 1 may exist in different forms due to removal of tandem repeats, or partial intraexonic splicing of RRBP1. The form presented here is lacking the canine p180 ribosome-binding domain, NQGKKAEGAQ, which is tandemly repeated close to the N-terminus in other forms that havent been fully characterized. RRBP1 has been excluded as a candidate gene in the cause of Alagille syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RRBP1 using anti-RRBP1 antibody (A07074-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human HepG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RRBP1 antigen affinity purified polyclonal antibody (Catalog # A07074-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RRBP1 at approximately 200KD. The expected band size for RRBP1 is at 152KD.
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