Western blot using GeneTexs Affinity Purified anti-Rif1 antibody shows detection of a band ~265 kDa corresponding to mouse Rif1 (arrowhead). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (data not shown). Approximately 25 microg of MEF whole cell lysate was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1.0 ug/ml for 2 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye800 conjugated goat anti-Rabbit IgG [H&L] MX for 45 min at room temperature. IRDye800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Western blot using GeneTexs Affinity Purified anti-Rif1 antibody shows detection of a band ~265 kDa corresponding to mouse Rif1 (arrowhead). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (data not shown). Approximately 25 microg of MEF whole cell lysate was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1.0 ug/ml for 2 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye800 conjugated goat anti-Rabbit IgG [H&L] MX for 45 min at room temperature. IRDye800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
RIF1 antibody
GTX48737
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetRif1
Overview
- SupplierGeneTex
- Product NameRIF1 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ELISA: 1:5000-1:40000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID51869
- Target nameRif1
- Target descriptionreplication timing regulatory factor 1
- Target synonyms5730435J01Rik; 6530403D07Rik; AU016181; AW549474; D2Ertd145; D2Ertd145e; mRif1; Rap1 interacting factor 1 homolog; rap1-interacting factor 1 homolog; telomere-associated protein RIF1
- HostRabbit
- IsotypeIgG
- Protein IDQ6PR54
- Protein NameTelomere-associated protein RIF1
- Scientific DescriptionRif1 (also known as telomere-associated protein RIF1 and Rap1-interacting factor 1) is a mouse ortholog of the yeast Rif1 family of telomere-associated proteins identified on the basis of its high expression in primordial germ cells and embryo-derived pluripotent stem cell lines. Mouse Rif1 is a protein involved in negative regulation of telomere length. It is also localized to ATM dependent DNA damage foci and may act at the chromatin level to allow access to some factors.
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Comprehensive nucleosome interactome screen establishes fundamental principles of nucleosome binding. Skrajna A et al., 2020 Sep 25, Nucleic Acids ResRead more
Datasheet
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