
IHC-P analysis of human kidney tissue using GTX85728 RIF1 antibody. Antigen retrieval : Heat-induced antigen retrieval Dilution : 5 microg/ml
RIF1 antibody
GTX85728
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetRif1
Overview
- SupplierGeneTex
- Product NameRIF1 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500 - 1:3000. IHC-P: 5 microg/ml. ELISA: 1:5000 - 1:40000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID51869
- Target nameRif1
- Target descriptionreplication timing regulatory factor 1
- Target synonyms5730435J01Rik; 6530403D07Rik; AU016181; AW549474; D2Ertd145; D2Ertd145e; mRif1; Rap1 interacting factor 1 homolog; rap1-interacting factor 1 homolog; telomere-associated protein RIF1
- HostRabbit
- IsotypeIgG
- Protein IDQ6PR54
- Protein NameTelomere-associated protein RIF1
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203


![Western blot using GeneTexs Affinity Purified anti-Rif1 antibody shows detection of a band ~265 kDa corresponding to mouse Rif1 (arrowhead). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (data not shown). Approximately 25 microg of MEF whole cell lysate was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1.0 ug/ml for 2 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye800 conjugated goat anti-Rabbit IgG [H&L] MX for 45 min at room temperature. IRDye800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](https://www.genetex.com/upload/website/prouct_img/normal/GTX48737/GTX48737_20160330_WB_w_23060823_556.webp)
