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WB analysis of HUVEC, COS, and Jurkat cell lysates using GTX87628 RIMKA antibody. The lane on the right is blocked with the synthesized peptide.
WB analysis of HUVEC, COS, and Jurkat cell lysates using GTX87628 RIMKA antibody. The lane on the right is blocked with the synthesized peptide.
WB analysis of HUVEC, COS, and Jurkat cell lysates using GTX87628 RIMKA antibody. The lane on the right is blocked with the synthesized peptide.

RIMKA antibody

GTX87628
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetRIMKLA
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Overview

  • Supplier
    GeneTex
  • Product Name
    RIMKA antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500~1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID284716
  • Target name
    RIMKLA
  • Target description
    ribosomal modification protein rimK like family member A
  • Target synonyms
    FAM80A, NAAGS, NAAGS-II, NAAGS2, N-acetylaspartylglutamate synthase A, N-acetylaspartyl-glutamate synthetase A, N-acetylaspartylglutamate synthetase II, N-acetylaspartylglutamylglutamate synthase A, NAAG synthetase A, RP11-157D18.1, family with sequence similarity 80, member A, ribosomal protein S6 modification-like protein A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8IXN7
  • Protein Name
    N-acetylaspartylglutamate synthase A
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Ji T, Pang Y, Cheng M, et al. mNSCs overexpressing Rimkla transplantation facilitates cognitive recovery in a mouse model of traumatic brain injury. iScience. 2023,26(10):107913. doi: 10.1016/j.isci.2023.107913
    Read this paper

Datasheet

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Figure 1. Western blot analysis of RIMKLA using anti-RIMKLA antibody (A11921-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RIMKLA antigen affinity purified polyclonal antibody (Catalog # A11921-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RIMKLA at approximately 43 kDa. The expected band size for RIMKLA is at 43 kDa.
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