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RIMKLA Antibody (90-170 aa, Internal)

LS-C383451
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetRIMKLA
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    RIMKLA Antibody (90-170 aa, Internal)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:20000), WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID284716
  • Target name
    RIMKLA
  • Target description
    ribosomal modification protein rimK like family member A
  • Target synonyms
    FAM80A, NAAGS, NAAGS-II, NAAGS2, N-acetylaspartylglutamate synthase A, N-acetylaspartyl-glutamate synthetase A, N-acetylaspartylglutamate synthetase II, N-acetylaspartylglutamylglutamate synthase A, NAAG synthetase A, RP11-157D18.1, family with sequence similarity 80, member A, ribosomal protein S6 modification-like protein A
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RIMKLA using anti-RIMKLA antibody (A11921-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RIMKLA antigen affinity purified polyclonal antibody (Catalog # A11921-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RIMKLA at approximately 43 kDa. The expected band size for RIMKLA is at 43 kDa.
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