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FACS analysis of HEK293T cells transfected with either RIT2 plasmid(Red) or empty vector control plasmid(Blue) using GTX83711 RIT2 antibody [4B5].
FACS analysis of HEK293T cells transfected with either RIT2 plasmid(Red) or empty vector control plasmid(Blue) using GTX83711 RIT2 antibody [4B5].
FACS analysis of HEK293T cells transfected with either RIT2 plasmid(Red) or empty vector control plasmid(Blue) using GTX83711 RIT2 antibody [4B5].

RIT2 antibody [4B5]

GTX83711
GeneTex
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
ReactivityHuman
TargetRIT2
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Overview

  • Supplier
    GeneTex
  • Product Name
    RIT2 antibody [4B5]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500. FACS: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    4B5
  • Concentration
    0.84 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6014
  • Target name
    RIT2
  • Target description
    Ras like without CAAX 2
  • Target synonyms
    RIBA, RIN, ROC2, GTP-binding protein Rit2, GTP-binding protein Roc2, Ric-like, expressed in neurons, ras-like protein expressed in neurons, ras-like without CAAX protein 2
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDQ99578
  • Protein Name
    GTP-binding protein Rit2
  • Scientific Description
    Binds and exchanges GTP and GDP.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Fagan RR, Kearney PJ, Sweeney CG, et al. Dopamine transporter trafficking and Rit2 GTPase: Mechanism of action and in vivo impact. J Biol Chem. 2020,295(16):5229-5244. doi: 10.1074/jbc.RA120.012628
    Read this paper

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Figure 1. Western blot analysis of RIT2 using anti-RIT2 antibody (A07833-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human SK-N-SH whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RIT2 antigen affinity purified polyclonal antibody (Catalog # A07833-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RIT2 at approximately 17,21 kDa. The expected band size for RIT2 is at 25 kDa.
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